![]() lncRNAs are the scientific frontier of the genome era and may reveal new RNA-mediated genetic expression regulatory networks, which could clearer clarify function of genome from the perspective of non-coding RNAs. Unlike mRNAs, the primary structure of lncRNAs are not highly conserved, and a considerable number of lncRNAs are transcripted from introns, exons, intergenic regions and overlapping regions 1. LncRNAs are non-coding RNAs of greater than 200 nt in length. The MSTRG.80946.2 was verified by network and experiments to be a key functional lncRNA under cold stress, regulating ACP1, TSPY1 and Tsn. lncRNAs played crucial roles in energy metabolism, growth and development, immunity and reproductive performance in cold stressed rats. To sum up, our work was the first to perform detailed characterization and functional analysis of cold stress-related lncRNAs in rats liver. Results confirmed the accuracy of our analysis. Finally, hub lncRNA MSTRG.80946.2 was characterized, and verified its relationship with related mRNAs by antisense oligonucleotide (ASO) interference and qRT-PCR. qRT-PCR confirmed the sequencing results. Functional analysis of key lncRNA targets showed that targets were significantly enriched in fatty acid metabolism, the PI3K-Akt signaling pathway and pathways in cancer under cold stress. We found the key lncRNA MSTRG.80946.2 in sub-network. To further reveal the mechanism of cold stress, DElncRNA and DEmRNA were extracted to reconstruct a co-expression sub-network. Next, a interaction network between lncRNA and its targets was constructed. GO and KEGG analysis revealed that lncRNA targets were mainly participated in the regulation of nucleic acid binding, cold stimulation reaction, metabolic process, immune system processes, PI3K-Akt signaling pathway and pathways in cancer. The target genes of DElncRNA were predicted by cis and trans, and then functional and pathway analysis were performed to them. 2,120 new lncRNAs and 273 differentially expressed (DE) lncRNAs were identified in low temperature environments. lncRNA libraries were constructed by high-throughput sequencing (HTS) using rat livers. Six SPF male Wistar rats were randomly divided to the acute cold stress group (4 ☌, 12 h) and the normal group (24 ☌, 12 h). Here, we built a cold stress animal model firstly. Although cold stress-related lncRNAs have been reported in plants, no research is available on the characteristic and functional analysis of lncRNAs after cold stress in rats. Long non-coding RNAs (lncRNAs) take part in many biological processes through transcriptional regulation, intracellular material transport, and chromosome remodeling. Cold stimulation reduces the quality of animal products and increases animal mortality, causing huge losses to the livestock industry in cold regions.
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